this product, a sheep anti bovine IgG1 HRP conjugated antibody, recognizes bovine IgG1.
No cross - reactivity with other bovine immunoglobulin classes is seen in immunoelectrophoresis.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/10,000 - 1/100,000
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antisera to bovine IgG1 were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.