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Wizard? SV Gel and PCR Clean-Up System and x-tracta? Gel Extractor Bundle

Wizard? SV Gel and PCR Clean-Up System and x-tracta? Gel Extractor Bundle

产品编号：A9283 品牌：promega 原厂货号：A9283
产品分类：DNA和RNA纯化 > DNA纯化 > PCR产物纯化及凝胶回收 > 胶回收
应用分类：

包装：

50 preps/25 extractors

运保温度:

室温

到货周期：

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1.含干冰类产品有运费；

2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

其它组分：

Wizard® SV Minicolumns【子货号：A129A，包装：1 x 50/pk，，运保温度：室温】

Collection Tubes【子货号：A130A，包装：1 x 50/pk，，运保温度：室温】

Membrane Wash Solution【子货号：A929B，包装：1 x 15ml，，运保温度：室温】

Membrane Binding Solution【子货号：A930B，包装：1 x 20ml，，运保温度：室温】

Nuclease-Free Water【子货号：P119A，包装：3 x 1,250μl，，运保温度：室温】

x-tracta™ Gel Extractor【子货号：A2121，包装：1 x 25/pack，，运保温度：室温】

描述：

说明

Wizard^® SV Gel and PCR Clean-Up System（Wizard^® SV凝胶及PCR纯化系统）用于从标准或低熔点琼脂糖凝胶提取并纯化长
度为100bp-10kb的DNA片段，这些DNA片段可直接来自PCR或其它一般的反应如限制性酶切反应。回收效率因DNA片段的大小而异，
可高达95%。PCR产物纯化可有效去除未掺入的核苷酸及引物。该系统利用膜可结合高达40μg的DNA，纯化DNA片段或PCR产物所需
的时间取决于处理样品的数量及操作步骤，可在15分钟内完成。纯化的DNA可直接用于自动荧光测序、克隆、标记、扩增、限制性酶
切及体外转录和翻译。

x-tracta™ Gel Extractor（x-tracta™ 切胶器）提供了一种方便安全的切胶方法，用于切下小块琼脂糖凝胶以进行后续实验。该工具可从琼脂糖凝胶上切下一片 0.13 × 0.33 英寸的胶块，可以方便地转移到离心管中进行后续实验。 x-tracta™工具使得切胶时不再需要使用刀片或小刀，其一次性使用的设计也消除了样品间交叉污染的可能性。

注意：x-tracta™ 切胶器在 0.6–2% 的分析级琼脂糖凝胶上最适用。如果将其用于低熔点（LMP）琼脂糖凝胶，请小心操作；由于低熔点琼脂糖凝胶稠度的问题，切胶器不能有效切割。

特点

• 高效率：可在 15 分钟内纯化 DNA 片段或 PCR 产物。

• 高回收率： DNA 片段回收率高达 95% ，反应体系可低达 15μl 。

• 质量保证：纯化的 DNA 用自动荧光测序可读取 700 个碱基，准确率 >98% 。

• 下游应用：纯化的 DNA 可直接用于自动荧光测序、克隆、标记、限制性酶切及体外转录 / 翻译等。

• 应用广泛：这一纯化系统可替换其他供应商的 4 个试剂盒。

原厂资料：

Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time Wizard® SV Gel and PCR Clean-Up Start-Up Kits are available. Purchase a Wizard® SV Gel and PCR Clean-Up System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, contact your Promega representative or an authorized Promega distributor.

The Wizard® SV Gel and PCR Clean-Up System is designed to extract and purify DNA fragments of 100bp to 10kb from standard or low-melting agarose gels or to purify products directly from PCR and other common reactions such as restriction digests. Up to 95% recovery is achieved depending upon the DNA fragment size. PCR products are commonly purified to remove excess nucleotides and primers. This membrane-based system, which can bind up to 40μg of DNA, allows recovery of isolated DNA fragments or PCR products in as little as 15 minutes, depending on the number of samples processed. The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation without further manipulation.

Features - Benefits

Improved Productivity: Purify DNA fragments or PCR products in as little as 15 minutes.
Enhanced Cloning Results: Up to 95% recovery eluted in as little as 15μl.
Confidence in Results: Purified DNA routinely achieves 700 bases with >98% accuracy in automated fluorescent sequencing.
Applications Tested: DNA is suitable for automated fluorescent sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation without further manipulation.
One System to Do It All: One system can replace up to four kits from other suppliers.

The x-tracta™ Gel Extractor tool provides a convenient, safe method for removal of agarose gel fragments for further processing. The device removes a 0.13 × 0.33 inch gel piece from agarose gels for easy transfer into a microcentrifuge tube for processing. The x-tracta™ tool eliminates the need for razor blades or scalpels, and its single-use design eliminates the possibility for sample-to-sample cross-contamination.

Note: The x-tracta™ Gel Extractor works best on 0.6–2% analytical grade agarose gels. Please exercise caution if using the x-tracta™ Gel Extractor on Low Melting Point (LMP) agarose gels because the extractor does not work effectively on these due to the gel consistency.