Wizard? Plus Megapreps DNA Purification System

• 产品编号：A7300      品牌：promega       原厂货号：A7300
• 产品分类：DNA和RNA纯化 > DNA纯化 > 质粒DNA纯化
• 应用分类：核酸分离纯化与分析 > DNA纯化 > 质粒 DNA 纯化

其它组分：

描述：

Wizard^® Plus Megapreps DNA Purification System(Wizard^® Plus巨量DNA纯化系统)提供简单、快速的大规模纯化质粒DNA的方法，无需氯化铯:溴化乙锭梯度离心。使用该系统仅需一个离心机、真空泵以及一个真空多联器。该系统通常在不到3个小时内，从1000ml的培养物中得到超过1 mg 的高拷贝数质粒DNA(200–20,000 bp)。纯化的DNA用无核酸酶的水(Cat.# P1193)或TE缓冲液洗脱，无需其它操作，就可直接用于自动荧光DNA测序及限制酶消化。也可用于体外转录反应，此时反应体系中需含有核糖核酸酶抑制剂(如重组RNasin^® Ribonuclease Inhibitor，Cat.# N2511)。

. 快速：DNA结合与离心柱洗涤均可批量处理，快速地完成。

• 安全：无需氯化铯:溴化乙锭梯度离心分离，且无需有机抽提。

• 可靠：得到可与氯化铯：溴化乙锭梯度技术相比较数量及质量的质粒DNA，但是后者需要更多的时间和劳动强度。

• 高产量：使用高拷贝数质粒时，每次巨量制备可从1000 ml细菌培养物中获得的DNA产量大于1mg。

• 高质量：DNA适合限制酶消化、自动荧光DNA测序、转化及亚克隆。

原厂资料：

Large-scale plasmid preparations, such as cesium chloride purification, can be both laborious and time-consuming, often requiring an overnight centrifugation. The Wizard® Plus Megapreps DNA Purification System is simple and rapid and requires only a centrifuge, vacuum source and vacuum manifold such as the Vac-Man® (20-sample capacity, Cat.# A7231) or Vac-Man® Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. The system yields up to one milligram of high-copy-number plasmid DNA (200–20,000bp) from a 1,000ml culture in less than three hours.
The Wizard® Plus Megapreps System does not require organic extractions or cesium chloride gradients. Purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) or TE buffer. The purified plasmid can be used directly for DNA sequencing and restriction digestion without further manipulation and can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).

注意事项：

储存于22-25℃。