Cell Resuspension Solution【子货号:A711C,包装:1 x 150ml,,运保温度:22-25℃】
Cell Lysis Solution【子货号:A712D,包装:1 x 150ml,,运保温度:22-25℃】
Neutralization Solution【子货号:A713C,包装:2 x 150ml,,运保温度:22-25℃】
Maxi/Megapreps Filtering System【子货号:A742D,包装:2 x 5 pack,,运保温度:22-25℃】
Wizard® Maxipreps DNA Purification Resin【子货号:A769A,包装:1 x 100ml,,运保温度:22-25℃】
Column Wash Solution【子货号:A810C,包装:1 x 125ml,,运保温度:22-25℃】
描述:
说明
Wizard® Plus Maxipreps DNA Purification System(Wizard® Plus大量DNA纯化系统)提供简单、快速的树脂式大量离心柱法纯化质粒DNA,无需氯化铯:溴化乙锭梯度离心。使用该系统仅需一个离心机、真空泵以及一个真空多联器。该系统通常在不到3个小时内,从100-500ml的培养物中得到300μg–1mg的高拷贝质粒DNA(200–20,000 bp)。纯化的质粒可直接用于自动荧光DNA测序及限制酶酶切消化,无需进一步操作,也可用于体外转录反应,此时反应体系中需含有核糖核酸酶抑制剂(如重组RNasin® Ribonuclease Inhibitor,Cat.# N2511)。
特点
.灵活:DNA适合限制酶消化、自动荧光DNA测序、转化及亚克隆。
• 高质量:得到的质粒DNA的产量和质量可与氯化铯:溴化乙锭梯度方法相媲美,但后者耗时费力。
• 快速:DNA结合与离心柱洗涤均可批量处理,快速地完成。
• 安全:无需氯化铯:溴化乙锭梯度离心分离,且无需有机抽提。
原厂资料:
The Wizard® Plus Maxipreps DNA Purification System provides a simple and rapid resin-based batch column method for purification of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. This system typically yields 300μg–1mg of high-copy-number plasmid DNA (200–20,000bp) from a 100–500ml culture in less than three hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) and can be used directly for DNA sequencing and restriction digestion without further manipulation. The DNA also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).
Features - Benefits
Flexible: DNA is suitable for restriction enzyme digestions, automated fluorescent DNA sequencing, transformation and subcloning.
High Quality: Yields plasmid DNA of comparable quantity and quality to cesium chloride:ethidium bromide gradient techniques that are much more time- and labor-intensive.
Fast: Rapid batch binding and column washing method used for DNA isolation.
Safe: Eliminates the need for cesium chloride:ethidium bromide gradient centrifugation and does not require organic extractions.