GoScript™ RT Mix for 1-Step RT-qPCR (50X) 当中包含 GoScript ™逆转录酶, RNasin® Plus RNA 酶抑制剂和其他组分,各组分含量均经过优化,以提高单步反应效果。
• 优越的性能:可在任何实时定量仪器进行灵敏的检测。
• 稳定性更高: 独特的室温组装使其适合用于自动化和高通量检测。
• 灵活:兼容快速和标准循环程序。
• 强劲:在抑制因子存在的情况下可高效合成全长 cDNA 。
• 可靠: Promega PCR 性能保证。
原厂资料:
The GoTaq® Probe qPCR Master Mix is optimized for quantitative PCR assays in the hydrolysis probe detection format. It is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). This master mix does not contain a reference dye; however, a separate tube of carboxy-X-rhodamine (CXR) reference dye is included with this system, allowing users to add reference dye to amplification reactions if desired. The GoTaq® Probe qPCR Master Mix is designed to provide resistance to a wide range of PCR inhibitors. This formulation uses antibody-mediated hotstart chemistry, allowing reaction setup to be performed at room temperature. The master mix also employs rapid hot-start activation and processive enzymes, making it compatible with both standard and fast instrument cycling programs.