The GoScript™ Reverse Transcription System(a,b) is a convenient kit that includes a reverse transcriptase and an optimized set of reagents designed for efficient synthesis of first-strand cDNA optimized in preparation for PCR amplification. The components of the GoScript™ Reverse Transcription System can be used to reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. Figure 1 provides an overview of the reverse transcription procedure. The optimized reaction buffer and reverse transcriptase provided in the GoScript™ Reverse Transcription System enable robust, full-length cDNA synthesis for reproducible analysis of rare or long messages.