GoScript? Reverse Transcription System

• 产品编号：A5000      品牌：promega       原厂货号：A5000
• 产品分类：PCR/RT-PCR/qRT-PCR > RT-PCR/RT-qPCR > RT-PCR（Endpoint）
• 应用分类：PCR > 反转录 > 反转录酶及试剂盒

其它组分：

描述：

原厂资料：

The GoScript™ Reverse Transcription System is a convenient kit that includes a reverse transcriptase and an optimized set of reagents for efficient synthesis of first-strand cDNA optimized in preparation for PCR amplification. The components of the GoScript™ Reverse Transcription System can be used to reverse transcribe RNA templates starting with either total RNA, poly(A)+ mRNA or synthetic transcript RNA. The optimized reaction buffer and reverse transcriptase provided in the GoScript™ Reverse Transcription System enable robust, full-length cDNA synthesis for reproducible analysis of rare or long messages. These conditions were developed for cDNA synthesis or for easy transition to gene-specific target amplification. Up to 5μl of the reverse transcription reaction can be directly amplified using Taq DNA polymerase in a 25µl PCR. GoScript™ Reverse Transcriptase is qualified for use in two-step RT-qPCR, using GoTaq® qPCR and Plexor® qPCR Systems. In two-step RT-qPCR using GoTaq® qPCR Master Mix, samples of the GoScript™ Reverse Transcription System can be added directly, up to 20% v/v.

Product Size Cat.#
GoScript™ Reverse Transcription System 50 reactions A5000
Each system contains sufficient reagents for 50 first-strand cDNA synthesis reactions of
20μl each.
• 50μl GoScript™ Reverse Transcriptase
• 300μl GoScript™ 5X Reaction Buffer
• 750μl MgCl2 (25mM)
• 200μl PCR Nucleotide Mix
• 50μg Oligo(dT)15 Primer
• 50μg Random Primers
• 1.25ml Nuclease-Free Water
• 2,500u Recombinant RNasin® Ribonuclease Inhibitor

The GoScript™ Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents for efficient synthesis of first-strand cDNA optimized for quantitative PCR amplification. GoScript™ Reverse Transcriptase uses M-MLV Reverse Transcriptase and state-of-the-art buffer technology to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors. GoScript™ Reverse Transcriptase is qualified for use in qPCR, including GoTaq® qPCR and Plexor® RT-qPCR systems.

Features - Benefits

• Ultra-Active: Save money on every reaction.
• Sensitive: Detect rare transcripts.
• Processive: Transcribe long messages.
• Resilient: Synthesize cDNA in the presence of strong inhibitors.

Applications

• RT-qPCR.
• RT-PCR.

注意事项：