Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Class
IgG
Cross Adsorption
Against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit and sheep IgG
Antibody Form
F(ab')2 Fragment
Tested Applications
Dilution *
ELISA (ELISA)
1:500-1:20,000
Immunohistochemistry (IHC)
1:500-1:20,000
Western Blot (WB)
1:500-1:20,000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Background/Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.