The Applied Biosystems® TaqMan® hPSC Scorecard™ Panel 384w enables verification of pluripotency and determination of lineage bias for both human ES and iPS cell lines. The 384-well plate contains four sets of 94 predefined TaqMan® Gene Expression assays (including endogenous controls) dried-down in the wells. Master mix is not included with this panel. For a kit that combines the panel with master mix, see TaqMan® hPSC Scorecard™ Kit 384w. This product is also available in a 2 x 96w Fast format.
• Assess the gene signature of four samples in one simple experiment • Compare results to a reference standard with the accompanying hPSC Scorecard™ Analysis Software • Identify lines with trilineage differentiation potential • Evaluate inductive differentiation specified to one germ layer • Access validated content for increased confidence in your results
Assess an Entire Gene Signature in One Experiment The TaqMan® hPSC Scorecard™ Panel helps save time by offering pre-plated assays in a stable and convenient dried-down format. Simply add TaqMan® Master Mix to your cDNA of interest and transfer to the 384-well plate with a multichannel pipette.
Compare Results to a Reference Standard The TaqMan® hPSC Scorecard™ Panel includes access to proprietary cloud-based analysis software that allows you to view and export graphs and tables of results, as well as generate reports. The software is compatible with seven Applied Biosystems® qRT-PCR systems and is available at no additional charge.
Identify Lines with Trilineage Differentiation Potential Include randomly differentiated embryoid body (EB) samples (differentiated for at least 7 days using standard EB methods) in your analysis and determine whether your lines are biased toward one or more of the three germ layers (endoderm, mesoderm, ectoderm).
Evaluate Inductive Differentiation Specified to One Germ Layer The panel includes over twenty markers for each of the three germ layers that have been shown to respond as expected to directed differentiation in monolayer culture. Quickly evaluate time course data, culture conditions and media formulations for your germ layer of interest with this one panel.
Access Validated Content The panel contents are based on published work (Bock et al., Cell 144, 439–452, 2011) and were validated against multiple human ES and iPS lines.
What You Get The TaqMan® hPSC Scorecard™ Panel 384w includes:
• One 384-well plate of 94 TaqMan® Gene Expression assays (4 gene sets per plate) • One optical plate cover
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Contents: One 384-well plate with four replicates of the 96-assay panel and one optical cover
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