This Estrogen Receptor (ER) Alpha Ligand Binding Domain (LBD) is a 62.80 kDa recombinant human protein (amino acids 282-595) expressed as a GST fusion protein in baculovirus-infected insect cells. It was expressed and purified in the absence of exogenous ligands. ER Alpha-LBD recombinant protein binds [3H]-estradiol with sub-nM affinity in hydroxylapatite assays and binds specifically to estrogen response element (ERE) sequences.
ER Alpha-LBD recombinant protein is: • Flexible—ideal for ligand binding and coregulator displacement assays developed in-house or partnered with our LanthaScreen® ER Beta Coactivator Assay or LanthaScreen® ER Alpha Competitive Binding kits • Ready to use—active, purified, and tagged with a GST • Convenient—available in bulk sizes
ER Alpha-LBD recombinant protein is manufactured under the strictest quality parameters. This product is guaranteed for 6 months after purchase. Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.