ESSENTIAL 6 MEDIUM KIT

产品编号：A1516401 品牌：invitrogen 原厂货号：A1516401
产品分类：细胞,细胞培养与转染试剂 > 无血清培养基 > 干细胞培养基
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包装：

运保温度:

Store at 2 to 8°C.

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描述：

Essential 6™ Medium is a feeder-free and xeno-free medium that supports the reprogramming of somatic cells and differentiation of human pluripotent stem cells (PSCs). The formulation is based on a medium originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson and published as "E8." Essential 6™ Medium has been proven to support somatic cell reprogramming using a variety of methods, including episomal vectors (2) and CytoTune™ (Sendai virus). In addition, Essential 6™ Medium can be used as a base for the formulation of custom media for the culture of PSCs.

Essential 6™ Medium enables:

• Reprogramming—does not contain TGFΒ, which has a negative effect on reprogramming efficiency
• Differentiation—does not contain bFGF, which inhibits embryoid body formation and differentiation
• Flexibility—provides a flexible format where the levels of TGFΒ and bFGF can be adjusted and additional components can be added to match a given application

Reprogramming
Essential 6™ Medium allows for defined and feeder-free reprogramming when used with bFGF. The formulation supports somatic cell reprogramming using a variety of methods, including episomal vectors (2) and CytoTune™ (Sendai virus), and is optimized to help ensure maximum cell health and pluripotency with minimal variability.

Differentiation
Essential 6™ Medium is a reliable and robust medium for the formation of embryoid bodies. Unlike other media used in PSC culture, Essential 6™ Medium does not contain bFGF or TGFβ, which inhibits embryoid body formation and differentiation.

Flexibility
Essential 6™ Medium is xeno-free and contains only the essential components needed for stem cell culture, minus bFGF and TGFΒ. This provides a basal medium that will ensure maximum cell health and pluripotency, while allowing levels of TGFΒ and bFGF to be adjusted and additional components to be added to match a given application.

Commercialized in partnership with Cellular Dynamics International.

注意事项：

1 × 500 ml bottle, Store at 2 to 8°C. Protect from light.