The pJTI™ R4 Exp CMV-TO EmGFP pA vector is a positive control vector for assessing retargeting efficiency when retargeting a Jump-In™ T-REx™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector included in the Jump-In™ parental kits) and after antibiotic selection, the EmGFP can be inducibly expressed with doxycycline and the successfully retargeted cells will fluoresce green.
Ensure the Success of Your Jump-In™ T-REx™ Retargeting Reactions Successful retargeting of Jump-In™ T-REx™ parental cell lines like the Jump-In™ T-REx™ HEK293 Kit is dependent on a variety of factors, such as:
• Transfection efficiency • Cell confluency • Antibiotic selection conditions • Quality and concentration of DNA • Retargeting vector to integrase vector ratio
We strongly recommend including a positive control retargeting reaction using the pJTI™ R4 Exp CMV-TO EmGFP pA vector in your Jump-In™ experiment, along with negative controls (no plasmid DNA, no integrase vector), so you can easily visualize the results and optimize the retargeting conditions.
原厂资料：
注意事项：
pJTI™ R4 EXP CMV-TO EmGFP pA vector (100 µg), store at -20 °C
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