Every day, more researchers are using tunable diode lasers for three- or four-color cytometry. Our AlignFlow Plus flow cytometry alignment beads are designed for diode laser—excited research, including the calibration and focusing of flow cytometers. These 6.0-µm BODIPY dye—labeled microspheres are excitable between 630 and 660 nm with emission in the range of 670-720 nm.