EXPI293 EXPRESSION SYSTEM KIT Starter Kit

产品编号：A14635 品牌：invitrogen 原厂货号：A14635
产品分类：蛋白质表达与纯化 > 蛋白质表达系统 > 哺乳动物表达系统
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包装：

运保温度:

Store all components at +2°C to +8°C

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描述：

The Expi293™ Expression System is a major advance in transient expression technology for rapid and ultra high-yield protein production from mammalian cells. It is based on high density culture of Expi293F™ cells in Expi293™ Expression Medium. Transient expression is powered by the cationic lipid-based ExpiFectamine™ 293 transfection reagent in combination with specialized transfection enhancers. All components work in concert to generate 2- to 10-fold higher protein yields than previous generation transient expression systems such as the FreeStyle™ 293 Expression System. Expression yields of >1 gram per liter of transfected culture have been demonstrated for some antibody and non-antibody proteins.

The Expi293™ Expression System provides:

• Rapid production of milligram to gram quantities of recombinant protein in mammalian cells
• High density cultures of Expi293F™ cells that result in more expressing cells per milliliter of culture and higher protein yields
• Native folding and mammalian post-translational modifications, such as glycosylation, of expressed proteins
• Easy, robust culture and transfection protocols that readily fit into current transient expression workflows
• Scalable expression that can be applied to cultures from<1 mL to >10 L, thus generating the amount of protein needed

The components included in the Expi293™ Expression System Kit are: 2 vials of frozen Expi293F™ cells, 1 liter of Expi293™ Expression medium, one ExpiFectamine™ 293 transfection kit sufficient to transfect 1 liter of culture, Opti-MEM® reduced serum media, and an antibody-expressing positive control vector. All components of the Expi293™ Expression System are also available for purchase separately.

注意事项：

2 vials frozen Expi293F™ cells
1 bottle 1000 mL Expi293™ Expression Medium
1 ExpiFectamine™ 293 transfection kit for 1 L of culture
1 vial Antibody Expressing Positive Control Vector
1 bottle 100 mL Opti-MEM°C Reduced Serum Medium

Store all components at +2°C to +8°C, except frozen cells are stored in liquid nitrogen