Wizard®Plus SV Minipreps DNA Purification System(Wizard®Plus SV 质粒小量纯化系统) 组分
原厂资料：
To improve the quality of plasmid DNA isolated from both EndA+ and EndA–strains of E. coli, the Wizard® Plus SV Minipreps DNA urification System includes an alkaline protease solution. Alkaline protease, originally identified as subtilisin Carlsberg, is solated from the bacterium Bacillus licheniformis(1). Approximately 250µg are added per sample at the end of the lysis step during the preparation of a cleared bacterial lysate to inactivate endonucleases. The alkaline protease also acts to nonspecifically degrade proteins, thus reducing the overall level of protein contaminants in the cleared bacterial lysate.
Alkaline protease is useful in this procedure, because it is optimally active at pH 9 and above, the conditions present during the alkaline lysis procedure. When the lysate is neutralized, alkaline protease activity is substantially reduced.
The DNA prepared by this procedure has been ested extensively in a range of molecular biology applications including fluorescent sequencing, restriction enzyme digestion and cloning.
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