BACMAM HISTONE H3K4ME2 AB KIT

• 产品编号：A14164      品牌：invitrogen停用       原厂货号：A14164
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞通路检测试剂盒
• 应用分类：

描述：

The BacMam Histone H3K4me2 Cellular Assay is a high-throughput screening (HTS) compatible TR-FRET assay for the interrogation of dimethylation at lysine 4 on histone H3 in a cellular format. Baculovirus-mediated gene delivery (BacMam) provides a convenient tool for the expression of GFP-Histone H3 fusion protein in your cell background of interest. Coupled with a terbium (Tb)-labeled site-specific antibody, the resulting cellular assay can be used to identify inhibitors of methyltransferases and demethylases acting on lysine 4 of histone H3.

The Kit Includes: 

• BacMam Histone H3 Reagent
• LanthaScreen® Tb-anti-histone H3K4me2 Antibody
• 6X LanthaScreen® Cellular Lysis Buffer
• Instrument Control Terbium TR-FRET kit

With the BacMam Histone H3K4me2 Cellular Assay Kit You Can:
• Use your cell background of choice with the portability of BacMam
• Identify more relevant inhibitors since the methyl transferases and demethylases are in their natural protein complexes
• Conserve precious cell samples with the miniaturizable homogenous assay format
• Improve data quality with the advantages of TR-FRET

Get More Physiologically Relevant Results 
The BacMam Histone H3K4me2 Cellular Assay allows the investigation of dimethylation at lysine 4 on histone H3 with your choice of cellular backgrounds including primary cells. This enables screening for potential inhibitors of methyl transferases and demethylases associated with Lys4 dimethylation in their natural complexes in a physiologically relevant cell type.

BacMam and LanthaScreen® Convenience Saves Sample and Time
Assays are run in a fully homogenous, addition-only format without any washing, lysate transfer, or separation procedures required for traditional methods such as Western blotting and ELISA, making them ideal for HTS applications. By using the same BacMam reagent for GFP-Histone H3, other histone H3 modifications (Ph, Me, Ac) can be monitored just by exchanging the Terbium (Tb) labeled antibody. In addition, the application of the LanthaScreen® technology includes all the advantages of TR-FRET detection including reduced data noise, less interference from fluorescent compounds, and high sensitivity, allowing the use of fewer cells than traditional Western or ELISA methods.

原厂资料：

注意事项：

The kit includes:
• BacMam Histone H3 Reagent
• LanthaScreen® Tb-anti-histone H3K4me2 Antibody
• 6X LanthaScreen® Cellular Lysis Buffer
• Instrument Control Terbium TR-FRET kit

Upon receipt:
Store the BacMam Histone H3 Reagent, 6X LanthaScreen® Cellular Lysis Buffer, and Instrument Control Terbium TR-FRET kit at 4°C

Store the LanthaScreen® Tb-anti-histone H3K4me2 Antibody at -20°C.