The pJTI™ R4 Dest CMV N-EmGFP pA vector allows a gene of interest to be expressed as an N-terminal Emerald Green Fluorescent Protein (EmGFP) fusion in a Jump-In™ parental cell line. When used in combination with a Jump-In™ parental cell line kit such as the Jump-In™ GripTite™ HEK293 cell line (A14150), an isogeneic stable cell line can be created with less effort and in less time than traditional cell engineering methods. In addition, Gateway® technology increases the efficiency of the cloning steps to introduce your GOI into the Gateway®-ready pJTI™ R4 Dest CMV N-EmGFP pA vector. The high retargeting efficiency, made possible by the R4 sites in the Jump-In™ parental cell line, allows the use of the isogenic pool for additional experiments without the need for clonal selection. Alternatively, the high retargeting efficiency allows for easy selection of a positive stable clone for expressing your gene of interest with an N-terminal EmGFP.
The Jump-In™ Vectors and Parental Cell Lines Let You:
• Quickly and efficiently develop stably engineered isogenic cell pools in about half the time compared to traditional cell engineering methods. • Utilize isogenic expression from a defined genomic locus as the ideal solution for comparative analysis of gene families, isoforms or orthologs. • Generate multiple cell lines in parallel due to the simplified work flow. • Easily access the Jump-In™ technology with the ability to generate an unlimited number of cell lines without complicated licenses or restrictions to interpret.
Save Time with Rapid and Efficient Generation of Engineered Cell Lines Using Gateway® technology, you can generate an expression construct for retargeting using the pJTI R4 DEST CMV N-EmGFP pA destination vector. In combination with a Jump-In™ parental cell line kit, the expression cassette is efficiently and specifically inserted into a genomic target R4 site resulting in the generation of functional cell pools in as little as 2 weeks without laborious clone isolation and analysis. Even generation of clonal cell lines can be done with reduced time and effort due to the high percentage of positive clones.
Expand Your Experimental Capabilities The pJTI™ R4 Dest CMV N-EmGFP pA vector along with a Jump-In™ parental cell line provide the ideal solution for cells and assays where transient engineering technologies are problematic, as well as for difficult to "engineer" cell lines. The kit also provides a convenient way to create target panels of gene families, isoforms, or orthologs. Genes coding for large proteins or multi-unit proteins are not a problem since the Gateway® destination vectors accept large inserts.
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