CSM MEDIA FOR MAV203 FOR YEAST 2L

• 产品编号：A13292      品牌：invitrogen       原厂货号：A13292
• 产品分类：细胞,细胞培养与转染试剂 > 微生物培养基 > 酵母培养基 > 酵母培养基
• 应用分类：细胞培养产品与服务 > 微生物培养 > 酵母培养

描述：

The CSM Media for Mav203 Yeast Cells is a specifically designed media for making agar plates that enable the growth of Saccharomyces cerevisae MaV203 cells. This product comes with enough reagents to prepare 2 liters of solid media with the following components per liter:

• CSM (without tryptophan): 0.74 g
• Yeast Nitrogen Base: 6.66 g
• Ammonium Sulfate: 5 g
• Glucose: 20 g
• Agar: 20 g

Compatible with All MaV203 Applications
The CSM Media is optimized for the GeneArt® High-Order Genetic Assembly System, including the recovery for transformations of vectors once they have been adapted to yeast using the GeneArt® High-Order Vector Conversion Cassette. The media can also be used to grow MaV203 competent yeast cells, both library and sub-cloning scale (cat# 11281-011 and 11445-012).

Plate Preparation Protocol
For each liter, use one pouch of CSM -Trp Agar Yeast Media minus Glucose and 1 bottle of 20% Glucose. Dissolve the contents of the pouch in 900ml of distilled water and autoclave for 20 minutes on liquid cycle. Allow the solution to cool to 55°C and then add one bottle of 20% Glucose (100ml). Mix well and pour plates.

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