The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen® Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of histones. BacMam provides a convenient genetic delivery tool for a GFP-Histone H3 fusion protein in the cell line of interest. This kit describes an HTS-compatible cellular immunoassay measuring phosphorylation of GFP-Histone H3 at Ser10.
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Kit contains Terbium-labeled anti-Histone H3 antibody, BacMam Histone H3 Virus, and 6X Lysis Buffer. Store as indicated in handling documentation.