Access RT-PCR System

• 产品编号：A1250      品牌：promega       原厂货号：A1250
• 产品分类：PCR/RT-PCR/qRT-PCR > RT-PCR/RT-qPCR > RT-PCR（Endpoint）
• 应用分类：PCR > RT-PCR > 一步法 RT-PCR

其它组分：

描述：

原厂资料：

The Access RT-PCR System is designed for the reverse transcription (RT) and PCR amplification of a specific target RNA from either total RNA or mRNA. This one-tube, two-enzyme system provides sensitive, quick and reproducible analysis of even rare RNAs. The system uses AMV Reverse Transcriptase (AMV RT) from Avian Myeloblastosis Virus for first-strand DNA synthesis and thermostable Tfl DNA polymerase from Thermus flavus for second-strand cDNA synthesis and DNA amplification. The Access RT-PCR System includes an optimized single-buffer system that permits extremely sensitive detection of RNA transcripts, without a requirement for buffer additions between the reverse transcription and PCR amplification steps. This simplifies the procedure and reduces the potential for contaminating the samples. In addition, the improved performance of AMV Reverse Transcriptase at elevated temperatures in the AMV/Tfl 5X Reaction Buffer minimizes problems encountered with secondary structures in RNA.

Features - Benefits

• Maximum Control: Separate tubes of each component allow you to control every step of the reaction. You can optimize Mg²⁺ and perform no-reverse transcriptase control reactions.
• Less Template: Detect message from as little as 1pg of total RNA or mRNA.
• No Buffer Additions Required: The AMV/Tfl 5X Reaction Buffer results in optimal enzyme activity without buffer additions between the reverse transcription and DNA amplification steps.
• Performance-Tested System: Promega PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Guarantee

PCR Satisfaction Guarantee

Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account.That's Our PCR Guarantee!

Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests preformed.

Applications

For more information, see the Protocols & Applications Guide.

References

1. Miller, K. and Storts, D.R. (1995) Promega Notes 53, 2–5.
2. Kaledin, A.S. et al. (1981) Biokhimiia 46, 1576–84.

注意事项：

A1250, For Research Use Only. Not for Use in Diagnostic Procedures.A1250 Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research, commercial or diagnostic applications without any license or royalty fees.

Storage Conditions: Store all system components at –20°C. For long-term
storage, the Positive Control RNA with Carrier may be stored at –70°C.
See the expiration date on the kit label.