Hibernate® is a CO2-independent nutrient medium for the maintenance of neural cells, tissue, and tissue slices. Hibernate®-A is the nutrient medium for adult and postnatal cells or tissue in ambient carbon dioxide levels; Hibernate®-E is the nutrient medium for embryonic cells or tissue in ambient carbon dioxide levels. The first application allows for longer period (up to 48 hours) maintenance of neuron viability in ambient CO2. This gives scientists greater convenience and control running bench-top live cell experiments, such as microscopy studies, flow cytometry, and other physiology studies. The second application of Hibernate® is for the preservation of viable brain tissue before processing for up to a month with refrigeration and maintenance of tissue slices.
• Optimized media systems for the viable storage of neural tissue and slices for up to 1 month at 2 - 8 °C - convenient systems that allow scientists to preserve tissue⁄slices before processing
• Maintenance of neuronal cells viability and functions at ambient CO2 level for up to 48 hours - makes conducting live-cell experiments possible without an expensive CO2 chamber adapted to instrument
• Manufactured under cGMP.
GIBCO® Hibernate®-A and Hibernate®-E are CO2-independent nutrient media for the maintenance of neural cells and preservation of tissue. It is supplied as 1X medium and mixing of Hibernate® with GIBCO® B-27® Supplement (50X) yields a complete medium.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use, unless otherwise stated.
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