MEMBRANEPROTM FXNAL PROT EXP COMBO KIT

产品编号：A11667 品牌：invitrogen 原厂货号：A11667
产品分类：蛋白质表达与纯化 > 蛋白质表达系统 > 膜蛋白表达系统
应用分类：行业及应用 > 药物研发 > 靶标和先导药物鉴定及验证 > G 蛋白偶联受体生物学G-Protein Coupled Receptor Biology (GPCR) > Membrane Protein Expression System > MembranePro™ Functional Protein Expression System
　　　　　蛋白质研究 > 蛋白质表达 > 膜蛋白表达 > 功能性膜蛋白表达系统

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描述：

For customers studying GPCRs and other membrane proteins, MembranePro™ is the ready-to-use system that delivers enriched, functional membrane proteins efficiently and reliably. Benefits of MembranePro™ include:

• Proteins are displayed on human (or other mammalian) cell membranes
• Cellular quality control and mammalian posttranslational processing helps produce functional protein
• Proteins bud off as lipoparticles and are easily collected from the culture medium
• Lipoparticles are enriched with expressed receptors

MembranePro™ workflow is less labor intensive in comparison to traditional production of membrane fractions. Briefly, the MembranePro™ protocol allows you to:

1. Clone your gene of interest using a TOPO® vector
2. Transfect with Lipofectamine™ 2000 and MembranePro™ Reagent into 293FT cells
3. Harvest lipoparticles 48h post-transfection, concentrate with MembranePro™ Precipitation Mix
4. Store lipoparticles at -80 C or proceed to downstream assays

MembranePro™ products are offered in two configurations – as an expression kit (TOPO® vector, Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent, 293FT cells) and as a support kit (Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent). The expression kit is only available in a 10 reaction size; the support kit is available in 10, 60 and 600 reaction sizes.

注意事项：

The kit contains:
•pEF6 V5-His TOPO® TA Vector Kit for at least 10 TOPO® cloning reactions (Store at -20°C)
•3 x10^6 293FT Cells (Store in liquid nitrogen)
•1 x 75 ml MembranePro™ Precipitation Mix (Store at room temperature)
•1 x 0.3 mg (1 mg⁄ml) MembranePro™ Reagent (Store at -20°C)
•3 x 0.75 ml Lipofectamine™ 2000 (Store at +4°C)
•Manuals