The PichiaPink™ Secreted Protein Expression Kit is a new recombinant protein expression system based on the yeast Pichia pastoris. The PichiaPink™ Secreted Protein Expression Kit gives you convenient and cost-efficient bioproduction from shake flask to 1000-liter production of your recombinant protein. The PichiaPink™ Secreted Protein Expression Kit comes with our new Pichia strains, the pPINK-HC and pPINK-LC vectors, and secretion signal sequences for production of your protein.
• Get started with secreted protein production in Pichia pastoris.
• Screen your Pichia transformants rapidly by auxotrophy and color selection.
• Reduce protein degradation with protease-deficient Pichia pastoris strains.
The PichiaPink™ Secreted Protein Expression Kit comes with:
• PichiaPink™ Secreted Protein Vector Kit (Cat. No. A11153)
• PichiaPink™ Expression Strain Set (Cat. No. A11154)
• PichiaPink™ Media Kit (Cat. No. A11156)
A New Way to Screen Pichia Transformants
The PichiaPink™ Secreted Protein Expression Kit uses adenine auxotrophy to select for Pichia transformants. The PichiaPink™ strains have the ade2 genotype and require complementation with the ADE2 gene to grow without adenine. Use of an auxotrophic marker also makes it easier to maintain transformants. No antibiotics are required to maintain your transformants.
In addition, you can screen for Pichia transformants by color. The untransformed PichiaPink™ strains appear as pink colonies while transformed PichiaPink™ strains appear as white colonies (Fig 1).
Secrete Your Proteins with the pPINKα-HC Vector
The PichiaPink™ Secreted Protein Expression Kit comes with the pPINKα-HC vector. The pPINKα-HC vector has the α-mating factor signal sequence built-in and allows your protein to be secreted into the media. Secreting your protein into the media makes harvesting and purifying your protein easier. The pPINKα-HC vector is built around the ADE2 gene for complementing the ade2-deficient Pichia strains. This vector uses the methanol-induced AOX1 promoter to express your protein. Further, the pPINKα-HC vector is designed to be integrated at high-copy-number in your Pichia transformants.
The PichiaPink™ Secreted Protein Vector Kit (Cat. No. A11153) can be ordered separately to refill the PichiaPink™ Secreted Protein Expression Kit.
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The PichiaPink™ Secreted Protein Expression kit consists of:
• The PichiaPink™ Secreted Protein Vector kit containing the high-copy number plasmid with the S. cerevisiae Alpha mating factor presequence to yield secreted protein expression.
• The PichiaPink™ Expression Strain set containing 4 glycerol strains
• The PichiaPink™ Media Kit containing 4 basic media.
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