TOP10 ONE SHOT KIT

• 产品编号：A11146      品牌：invitrogen       原厂货号：A11146
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 哺乳动物表达载体
• 应用分类：

描述：

The ViraPower™ HiPerform™ Promoterless Gateway® Vector Kit contains the MultiSite Gateway®-adapted ViraPower™ HiPerform™ promoterless lentiviral expression vector, pLenti6.4⁄R4R2⁄V5-DEST™ for easy recombination-based cloning and lentiviral-based high-level expression of a target gene from any promoter of choice in dividing and non-dividing mammalian cells. The pLenti6.4⁄R4R2⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages
• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy, simultaneous, recombination-based cloning of multiple DNA fragments in a defined order and orientation using MultiSite Gateway® technology
• Expression of the target gene under the control of a promoter of choice
• Stable, long-term expression
• Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems

Key Features
• WPRE from the woodchuck hepatitis virus, increases transgene expression and cPPT from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
• Promoterless vector to express the target gene under the control of a promoter of choice
• Blasticidin selection marker for stable selection under control of PGK promoter for long-term, persistent expression

Kit includes
• pLenti6.4⁄R4R2⁄V5-DEST™ Kit
• pENTR™ 5’ TOPO® TA Cloning Kit (Cat # K59120)
• One Shot® Stbl3™ Chemically Competent E. coli (Cat # C737303)
• pENTR® Gus positive control plasmid

 

原厂资料：

注意事项：

The ViraPower™ HiPerform™ Promoterless Gateway® Vector Kit includes the following components:

• pLenti6.4⁄R4R2⁄V5-DEST™ Kit which includes:
 

Storage
• pLenti6.4⁄R4R2⁄V5-DEST™ Kit: -20°C
• pENTR™ 5’ TOPO® Reagents: -20°C
• One Shot® Stbl3™ Chemically Competent E.coli: -80°C
• One Shot® TOP10 Chemically competent E.coli: -80°C
• pENTR™ Gus positive Control: -20°C

• 6 µg of pLenti6.4⁄R4R2⁄V5-DEST™ vector (40 µL of vector at 150 ng⁄µL in TE Buffer, pH 8.0)
• 10 µg of pLenti6.4⁄CMV⁄V5-MSGW⁄lacZ plasmid (20 µL of vector at 0.5 µg⁄µL in TE Buffer, pH 8.0)
• 10 µg of pENTR 5’⁄CMVp plasmid (20 µL of plasmid at 0.5 µg⁄µL in TE Buffer, pH 8.0)
• 10 µg of pENTR 5’⁄EF1αp plasmid (20 µL of plasmid at 0.5 µg⁄µL in TE Buffer, pH 8.0)

• pENTR 5’ TOPO® TA Cloning Kit (Cat # K95120) which includes:

• pENTR 5’ TOPO® Reagents
• One Shot® TOP10 Chemically Competent E.coli (Cat # C404003)
• One Shot® Stbl3™ Chemically Competent E. coli (Cat # C737303): 21 X 50 µL of Stbl3™ Cells, 6 mL of S.O.C. Medium and 50 µL of pUC19 control DNA (10 pg⁄µL)
• pENTR™ Gus positive control plasmid