Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces alboniger. It inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. This antibiotic also inhibits the growth of Gram positive bacteria and various animal and insect cells. Resistance to Puromycin is conferred by the expression of the "pac" gene. It is widely used in cell biology as selective agent in mammalian and stem cell culture systems.
Puromycin is stable for one year as solution when stored at -20°C. The recommended dose is at a range of 10-100 µg⁄ml, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg⁄ml. It acts quickly and can kill up to 99% of nonresistant cells within 2 days. When performing your experiment, we reccomend you to determine the optimal concentration of antibiotic required to kill your host cell line by performing a dose-response curve.
Formula: C22H29N7O5, 2HCl Formula Weight: 544.3 g⁄mole. Format: Liquid Solution (Sterile Filtered), 查看更多10mg⁄mL Volume: 10 x 1mL
京公网安备11010802025653 版权所有：北京逸优科技有限公司
