Bac-to-Bac® Baculovirus Expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. It relies on generation of recombinant virus by site-specific transposition in E. coli rather than homologous recombination in insect cells. New: pFastBacTOPO® vectors!
Reliable and fast protein expression
The Bac-to-Bac® Baculovirus Expression System is faster and easier than traditional baculovirus expression, and it maintains high levels of protein expression. It is based on site-specific transposition in E. coli rather than homologous recombination in insect cells. The Bac-to-Bac® Baculvirus Expression System is highly regarded in academic Literature. At least 80 citations every year since 2000!
Traditional baculovirus systems require purification and amplification of an initial low-titer viral supernatant. This requires a time-consuming plaque assay. Bac-to-Bac®'s pFastBac™ vector, however, recombines with the parent bacmid in DH10Bac™ E. coli competent cells to form an expression bacmid. Transfect the bacmid into insect cells for fast production of a high titer of pure recombinant baculovirus particles in the very first transfection. You'll save weeks of precious time. Collect a pure P2 baculovirus stock on Day 10 without the necessity of tedious plaques assay (See Figure 1)
High expression, easy screening
pFastBac™ uses the strong polyhedrin promoter to generate high levels of expression in a variety of insect cell lines such as Sf9, Sf21, and High Five™ cells.
New additions:
Bac-to-Bac®'s pFastBac vectors are now available as pFastBacTOPO® vectors too. pFastBacTOPO® vectors are Blunt® TOPO® vectors and are supplied with Mach1™-T1R E. coli for easy cloning and pFastBac⁄GOI propagation. The new vectors enable you to:
• Amplify your gene of interest (GOI) with a PCR enzyme of highest fidelity such as Accuprime™ Pfx SuperMix.
• TOPO® clone the blunt-end PCR product into the new vector in only 5 minutes!
• Visualize colonies 8 hours after plating on ampicillin selective plates because Mach1™-T1R E. coli cells have a faster doubling time compared to other standard cloning strains.
• With the Bac-to-Bac® N-His TOPO® cloning or expression kit, you can produce a N-terminal His-fusion protein with a TEV cleavage site to purify with nickel-chelating resin (Invitrogen’s ProBond™ Purification System) and generate a native protein with the aid of Invitrogen’s popular AcTEV™ Protease.
The new vectors combine the TOPO® cloning technology with the highly regarded Bac-to-Bac® baculovirus expression features to enable easy cloning and high-level protein expression.
The pFastBacTOPO® vectors are offered as cloning and expression kits.
原厂资料：
注意事项：
Kit content for Bac-to-Bac® C-His TOPO® cloning kit (For details see the manual)
• Vector kit for 20 topo cloning reactions:
o pFastBac⁄CT-TOPO® Vector containing the C-terminal TEV cleavage sit and His-Tag.
o pFastBac⁄CT-Gus Control Plasmid (Control expression vector)
o Other reagents supplied: 10x PCR, dNTP Mix, Salt Solution, Sterile Water, Control PCR template, Polyhedrin Forward Primer, SV40 pA Reverse Primer
• Kit containing competent cells (20 reactions):
o One Shot® Mach1-T1R Chemically Competent E. coli
Vector kit: store at -20°C
One Shot® Mach1-T1R Chemically Competent E. coli: store at -80°C
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