RapidDirect Primary Antibody polyHRP IP/Western Blot Kit (with goat anti-rabbit antibody beads)

• 产品编号：A-9403-001B      品牌：solulink       原厂货号：A-9403-001B
• 产品分类：生化和细胞分析 > 标签、标记试剂盒 > 抗体、蛋白标记试剂盒
• 应用分类：

描述：

For IP/western blots. Modify your primary antibody directly with HRP. No purification. No secondary antibodies needed. Ideal for those who use the same antibody for multiple western blots with the additional benefit of time savings.

Solulink's RapidDirect Primary Antibody polyHRP IP/Western Blot Kit advantages:

• Produce a blot result with minimal background
• Eliminate secondary antibody incubation steps, thereby reducing the time required to complete the protocol
• Eliminate the need for secondary antibodies and therefore eliminate any cross-species contamination

Workflow Diagram
 

Solulink's RapidDirect Primary Antibody polyHRP IP/Western Blot Kit uses direct labeling of HRP to the anti-antigen antibody in near quantitative yield, producing a highly sensitive one-step detection reagent. This eliminates the root cause of the interfering bands resulting from binding of detecting reagents to heavy and light chains of antibodies and other proteins on the blot membrane.

This kit converts 75-100 µg of primary antibody to primary antibody polyHRP conjugate, requiring only pipettes and a microcentrifuge in <30 minutes hands-on-time. The final conjugate allows at minimum development of 10-40 western blots dependent on the inherent affinity of the primary antibody. Examples of western blots from both mouse LLC and cell lysates are presented in the manual.

原厂资料：