No chromatography required Solulink’s R-PE-Antibody All-in-One Conjugation Kit offers an innovative catalyzed TurboLink™ conjugation based on Solulink’s proprietary linking technology that delivers 100% conversion of antibody to conjugate and novel purification that generates conjugate free of unlabeled antibody or enzyme at a high final yield of >75%, withoutHPLC.The kit includes everything needed to make two direct-labeled R-PE antibody conjugates from 100 µg of any user-supplied antibody.
S-HyNic (succinimidyl-6-hydrazino-nicotinamide) linker, an NHS-ester, reacts with lysine residues, incorporating HyNic functional groups (hydrazinonicotinamide) onto the antibody.
Sulfo-S-4FB (succinimidyl-4-formylbenzamide) linker is conjugated to R-PE, providing a pre-activated, highly fluorescent R-PE called 4FB-R-PE.
HyNic-modified antibody is incubated with pre-activated 4FB-R-PE in the presence of the TurboLink™ catalyst, aniline, leading to rapid and efficient conversion of the antibody to conjugate through formation of stable bis-arylhydrazone bonds.
Catalyzed conjugation. Novel purification. The efficiency of catalyzed conjugation greatly simplifies conjugate purification. Solulink’s proprietary linking technology is catalyzed by the TurboLink™ catalyst, aniline, under mild reaction conditions, increasing the rate and efficiency, leading to quantitative conversion of free antibody to conjugate. The complete absence of free antibody at the end of the catalyzed reaction leaves only two components; excess R-PE and conjugate. After conjugation, a novel Q spin filter is used that quantitatively removes excess R-PE to provide high-purity, ready-to use conjugate.Purified conjugate is then eluted from the filter membrane, free of residual antibody and R-PE in high yield (75 μg).
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