9590-1222 is specific for vitronectin and will react with the native and denatured forms. It does not cross react with fibronectin.
Vitronectin is one of the 2 most important cell adhesive proteins found in the blood and extracellular matrix. It exists as a mixture of 2 monomeric glycoproteins that mediate cell adhesion through an interaction between the R-G-D sequence, present in the cell binding domains, and several different integrin receptors. It is also known as a complement lysis inhibitor and as a scavenger protein for macromolecular products of the complement and homeostasis cascades.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
5 µg/ml - 20 µg/ml
Immunohistology - Frozen
5 µg/ml - 20 µg/ml
Western Blotting
5 µg/ml - 20 µg/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.