Description: This Human IL-8 Ready-SET-Go! ELISA Set contains the necessary reagents, standards, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA). This ELISA set is specifically engineered for accurate and precise measurement protein levels from samples including serum, plasma, and supernatants from cell cultures. This second generation kit has increased sensitivity with a range from 2-250 pg/mL.
Human IL-8 (CXCL8), is a pro-inflammatory CXC chemokine. It is synthesized as a 99 amino acid precursor protein that is further processed into one of four isoforms, with the most common being 72 or 77 amino acids in length. IL-8(77) is secreted primarily by endothelial cells and is thought to be a less potent neutrophil activator than the other forms. It is present at high levels during fetal development, where it mediates angiogenesis rather than inflammation. The predominant form present in adults is IL-8(72), which is expressed by monocytes, macrophages, epithelial cells, and fibroblasts in response to inflammatory stimuli, environmental stress, and steroid hormones. IL-8(72) is essential for the activation and recruitment of neutrophils to sites of inflammation, and has also been found to influence T cell migration. Signaling occurs through the G-protein coupled receptors CXCR1 or CXCR2. IL-8 transcripts are often upregulated in tumors, and it is associated with tumor angiogenesis and metastasis.
Components
Capture Antibody. Pre-titrated, purified antibody Detection Antibody. Pre-titrated, biotin-conjugated antibody Standard. Recombinant protein for generating standard curve and calibrating samples Coating Buffer. 10X PBS ELISA Coating Buffer Assay Diluent. 5X Concentrated Detection Enzyme. Pre-titrated Avidin-HRP Substrate Solution. Tetramethylbenzidine (TMB) Solution Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards. 96-well Plates. Corning Costar flat-bottom plates (included with Cat. #s ending in -22 and -86)