Description: Interleukin-9 (IL-9) is a proinflammatory cytokine historically believed to be involved in type 2 immune responses. However, recent evidence suggests IL-9 may be secreted by a distinct T Helper lineage called Th9. These cells can either be derived from Th2 cells with TGF-β or differentiated directly from naïve CD4+ T cells with TGF-β and IL-4.
IL-9 is a member of the common cytokine receptor γ chain-dependent family of cytokines which also includes IL-2, IL-4, IL-7, IL-15 and IL-21. Its pleiotropic effects on Th2 lymphocytes, B lymphocytes, mast cells, eosinophils and gut and airway epithelial cells have implicated IL-9 in asthma and other allergy-related disorders.
This human IL-9 ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of human IL-9 protein levels from samples including serum, plasma, and supernatants from cell cultures.The assay demonstrates parallelism in measuring recombinant and native human IL-9 proteins with a standard curve range of 1 pg/ml to 100 pg/ml.
Components
Capture Antibody. Pre-titrated, purified antibody Detection Antibody. Pre-titrated, biotin-conjugated antibody Standard. Recombinant cytokine for generating standard curve and calibrating samples ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20). Assay Diluent. 5X concentrated Detection enzyme. Pre-titrated Avidin-HRP Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards 96 Well Plate. Corning Costar 9018 plates (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)
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Note: Please use eBioscience Cat. No. 39-8959 as ELISA standard if need additional vial of standard. eBioscience Cat. No. 14-8958 is not suitable for ELISA.