Description: This mouse IL-22 ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of mouse IL-22 protein levels from samples including serum, and supernatants from cell cultures. The assay demonstrates parallelism in measuring recombinant and native mouse IL-22 proteins and a standard curve range of 8 to 1000 pg/ml. Human IL-22 receptor at levels above 4ng/ml and IL-22 binding protein at level above 3ng/ml interfere with the assay, which suggest that the assay mainly detect the free IL-22.
IL-22 is a 20 kDa member of the IL-10 cytokine family that is secreted primarily by Th17 cells. IL-23 and IL-6 can directly stimulate naive T cell to produce IL-22. Some NK subset cells, so called NK22, have also been shown to produce IL-22 in respose to IL-23 stimulation. In in vitro Th17 cultures, induction of IL-22 expression is greater in response to IL-23 than IL-6 or TGF-ß, suggesting that this cytokine may be secreted by more fully differentiated Th17 cells in vivo. Recently it is reported that self antigen selected Th17 cells in thymus are recruited to the lung, gut and liver. These cells secrete IL-22 in response to self antigen and mediated host protection during inflammation. Elevated IL-22 serum levels have also been reported in the mouse acute hepatitis model.
Components
Capture Antibody. Pre-titrated, purified antibody Detection Antibody. Pre-titrated, biotin-conjugated antibody Standard. Recombinant cytokine for generating standard curve and calibrating samples Coating Buffer. 10X PBS ELISA Coating Buffer Assay Diluent. 5X concentrated Detection enzyme. Pre-titrated Avidin-HRP Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards 96 Well Plate. NUNC Maxisorp flat-bottom (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)