Human IL-35 ELISA Ready-SET-Go!, 2 plates (w/plates)

• 产品编号：88-7357-22      品牌：eBioscience       原厂货号：88-7357-22
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
• 应用分类：

描述：

Description: This Human IL-35 Ready-SET-Go! ELISA Set contains the necessary reagents, standards, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA). This ELISA set is specifically engineered for precise measurement of relative protein levels from samples including serum, plasma, cell lysates, and supernatants from cell cultures.The use of an EBI3-specific capture antibody and a p35-specific detection antibody renders this sandwich ELISA exquisitely specific for human IL-35.

Interleukin-35 (IL-35) is the most recently discovered member of the IL-12 subfamily of cytokines and is a heterodimer of the subunits EBI3 and p35. EBI3 is homologous to p40, and can be secreted independently as a monomer or homodimer, or along with p28 as the IL-27 cytokine. The p35 subunit was initially identified in association with p40 as the IL-12 cytokine, although the independent regulation of p40 and p35 expression led to speculation that these subunits could potentially associate with other proteins. This possibility was confirmed by the discovery of secreted EBI3 and p35 heterodimers in co-transfected cells.

Since its identification, the biology of the IL-35 heterodimer has remained incompletely understood, although it is clearly unique among the IL-12 family in expression and function. Early functional experiments demonstrated its ability to induce expansion and IL-10 production in CD4+ CD25+ Foxp3+ regulatory T cells and suppress the proliferation of Th17 cells, suggesting a role in immune regulation. This was further supported by the detection of IL-35 in supernatant from mouse regulatory, but not effector, T cell cultures by coprecipitation of the EBI3 and p35 subunits. However, attempts to produce similar results in natural, Foxp3+ human Treg were unsuccessful, suggesting that IL-35 had distinct functions in human and mouse biology. Later studies contradicted this by demonstrating that that inducible Treg, which are derived from mature effector T cells under certain stimulatory conditions, were capable of producing IL-35 after exposure to dendritic cells co-cultured with human rhinovirus (R-DC), and that the secreted IL-35 contributed to their immunosuppresant function. Additional research supported the findings that inducible Treg were a source of IL-35 and also indicated that IL-35 may play a role in the conversion of effector T cells to a suppressive phenotype. These findings provide further evidence that IL-35 functions mainly as an immunosuppressive cytokine.

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