收藏本站
热门搜索: A3500  E1910  A1360  25200056  dmem  

MO IL-23 UNCOATED ELISA

 
包装: 2X96T PLT
运保温度: 2° to 8°C
到货周期: 登录后查看
标准价: ¥客户可见
会员价: ¥客户可见
积分: 客户可见
 
登录之后可加入购物车购买,请您 
对比 收藏

运费与支付说明:

1.含干冰类产品有运费;

2. 必须现金付款或有信用额度的会员才可以直接发货,否则需要等待现金付款信息。

描述:

Description: This Mouse IL-23 (p19/p40) ELISA Ready-SET-Go! 'IS' ELISA Set (Improved sensitivity assay with 5B2 capture antibody) with high affinity binding ELISA plates contains the necessary reagents, buffers and diluents for performing quantitative, enzyme-linked immunosorbent assays (ELISA). The 5B2 antibody in combination with the C17.8 antibody yields an improved IL-23 sandwich ELISA with greater sensitivity than was achievable with previous assays. This ELISA reagent set is specifically engineered for accurate and precise measurement of mouse IL-23 protein levels from samples including serum, and supernatants from cell cultures. The assay demonstrates parallelism in measuring recombinant and native mouse IL-23 proteins with a standard curve range of 4 pg/ml to 500 pg/ml and assay sensitivity below 4 pg/ml. Native mouse IL-23 was detected in supernatants from bone marrow-derived, LPS-activated dendritic cells. The use of a p19-specific capture antibody and a p40-specific detection antibody renders this assay exquisitely specific for mouse IL-23. IL-12 p40 homodimer and IL-12 p70 were each run in the assay at 500 ng/ml with no interference or cross-reactivity observed. A panel of 20 unrelated cytokines was run in the IL-23 ELISA at 100 ng/ml with no cross reactivity observed; all values were at the limit of detection of the assay.
For measurement of total p40 protein levels, the Mouse IL-12/23 Total p40 ELISA Ready-SET-Go! Is available (88-7120).

 

Components Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 flat-bottom (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)

 


相关图片

说明书

参考文献

本产品可用于的实验

购此产品的人还购买了
最近浏览产品

京ICP备15036693号-2    京公网安备11010802025653    版权所有:北京逸优科技有限公司       0.15

 纠 错