Description: IgG1 comprises 60-65% of the total main subclass IgG, and is predominantly responsible for the thymus mediated immune response against proteins and polypeptide antigens. IgG1 binds to the Fc-receptor of phagocytic cells and can activate the complement cascade via binding to C1 complex. A deficiency in IgG1 isotype typically is a sign of a Hypogammaglobulinemia.
Components
Capture Antibody Concentrate
Detection Antibody Concentrate
Human IgG1 isotype control (standard), lyophilized, 20 ug/ml upon reconstitution
Phosphate Buffered Saline Concentrate (PBS)
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
Tetramethylbenzidine (TMB) Substrate Solution
96-well plate