8365-1256 is specific for alpha- spectrin/alpha-fodrin of all mammalian non-erythroid cells.
8365-1256 reacts with the 240 kDa full length alpha-fodrin molecule, the 150 kDa N-terminal cleaved product and the 120 kDa C-terminal product.
Species Cross-Reactivity
Target Species
Cross Reactivity
Guinea Pig
Yes
Human
Yes
Mammals
Yes
Mouse
Yes
Pig
Yes
Rabbit
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Chromatin Immunoprecipitation
Immunofluorescence
1/200 - 1/400
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/500 - 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Immunohistology
Does not work on paraffin sections.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.