The CaptureSelect™ VIIISelect Leakage ELISA detects VIIISelect affinity ligand contamination of B domain-depleted recombinant Factor VIII purified using VIIISelect affinity resin (GE Healthcare). Even when covalently attached, small amounts of the VIIISelect affinity ligand can leach off of the chromatography support and co-elute with human Factor VIII. This ELISA provides sensitivity to detect VIIISelect affinity ligand contamination of less than 1 ng/mL. This assay is designed to minimize interference and to provide accurate quantitation in the presence of human Factor VIII. The CaptureSelect™ VIIISelect Leakage ELISA can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.
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For Research Use Only. Not for use in diagnostic procedures.