8040-0106 is purified RNase A isolated from bovine pancreas. RNase A is an endoribonuclease that exists as a single chain polypeptide containing 4 disulphide bridges. It can be inhibited by alkylation of the histidine-12 or histidine-119 that are present in the active site. Activators of RNase A include potassium and sodium salts.
The optimal conditions for activity are 60ºC and pH 7.6.
CAS number: 9001-99-4
EC number: 3.1.27.5
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
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