Respiratory syncytial virus (RSV) is a 100-350 enveloped virus belonging to the Paramyxoviridae, genus Pneumovirus, that causes minor respiratory infections in adults and bronchitis and bronchopneumonia in children. Its genome consists of single stranded, negative sense RNA that codes for 2 non-structural and 8 structural proteins.
7950-1705 recognizes the RSV phosphoprotein, fusion and nuclear protein and reacts with human respiratory syncytial virus subtypes A and B.
It does not react with tissue culture isolates of influenza types A and B, parainfluenza types 1, 2, 3 & 4b, adenovirus, HSV types1 & 2, VZV, cytomegalovirus, mumps and measles virus, coxsackie B4 virus, poliovirus types 1, 2 & 3 or Echo virus 19.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunofluorescence(1)
1/5 - 1/20
Immunohistology - Frozen
1/50 - 1/400
Immunohistology - Paraffin(2)
1/50 - 1/400
(1)
Acetone fixation is recommended.
(2)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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