7950-0304 is specific for respiratory syncytial virus (RSV) a negative-sense, single-stranded RNA virus and member of theParamyxoviridaefamily. RSV causes respiratory tract infections in patients of all ages, but particularly affects infants and the immunosuppressed.
RSV encodes three envelope glycoproteins, a small hydrophobic (SH) protein of unknown function, a glycoprotein (G) known as the attachment protein, and a fusion (F) protein. The F protein directs fusion of viral and cellular membranes, resulting in viral penetration, and can lead to the formation of syncytia.
The F protein is thought to be the principal antigen responsible for inducing an immune response.
7950-0304 does not react with Parainfluenza 1-3, Influenza A and B, Adenovirus or uninfected HEp-2 or WI-38 cells. It is neutralizing and reacts well with bovine isolates.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/200 - 1/1000
Immunofluorescence
1/20 - 1/200
Immunohistology - Frozen
1/20 - 1/200
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.