7835-1009 recognises the lower molecular weight subunit (26 kD) of protein 14-3-3.
The 14-3-3 family consists of 30kD proteins that are involved in multiple cellular processes including protein kinase signalling pathways, regulation of cell cycle progression, transcription and intracellular trafficking. Protein interactions with 14-3-3 show distinct preferences for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Western Blotting
1/10 - 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to 14-3-3 were raised by repeated immunisations of sheep with highly purified antigen.
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