7820-0330 is specific for human prostate specific antigen (PSA), a 34 kDa serine protease produced by prostatic epithelial cells, which is also known as kallikrein 3. Serum PSA levels have been correlated with the stage of prostatic cancer, and have been used to monitor residual tumour levels and disease progression.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Western Blotting
In Western blotting a PSA triplet is observed at ~30 kDa in the absence of DTT and a doublet in the presence of DTT.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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