Protein A is a 45 kDa MSCRAMM (microbial surface components recognizing adhesive matrix molecules) surface protein originally found in the cell wall of the bacteriaStaphylococcus aureus.It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR.
Protein A binds the Fc region of various mammalian IgG subclasses including:
Human
IgG1, IgG2, IgG4
Mouse
IgG2a, IgG2b, IgG3
Rat
IgG1, IgG2c
Guinea pig
IgG1, IgG2
Rabbit
IgG
Dog
IgGA, IgGB, IgGC, IgGD
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Health And Safety Information
(A full Health and Safety assessment is available upon request)
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