CaptureSelect™ Biotin Anti-Free LC-kappa (Hu) Conjugate consists of a 13 kDa llama antibody fragment (affinity ligand) that binds highly selectively to free human kappa light chains and dimers thereof. The functionality is based on recognition of an epitope on the human CL-kappa domain that is masked in intact antibodies and Fab fragments. The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.
The CaptureSelect™ Biotin Anti-Free LC-kappa (Hu) Conjugate allows you to:
• Detect, quantitate, and characterize free human kappa light chains in (crude) antibody preps
• Avoid cross-binding with human kappa light chains present in intact and properly folded antibodies and Fab fragments of any human antibody isotype such as IgG, IgA, IgM, IgD, and IgE
Applications for CaptureSelect™ Biotin Anti-Free LC-kappa (Hu) Conjugate include ELISA, Western blot, Gyrolab®-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore® and IBIS-MX96 systems) and bio-layer interferometry (ForteBio® Octet® systems).
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注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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