CaptureSelect™ Biotin Anti-C-tag Conjugate consists of a 13 kDa camelid antibody fragment (affinity ligand) with high affinity and selectivity for the 4-amino-acid "C-tag" peptide tag E‑P‑E‑A (glutamic acid - proline - glutamic acid - alanine). The Biotin Anti-C-tag affinity ligand recognizes the E-P-E-A peptide when this tag is fused directly to the C-terminus of a protein. The affinity ligand—constructed as a mono-specific bihead—is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.
The CaptureSelect™ Biotin Anti-C-tag Conjugate allows you to:
• Detect, quantitate, and characterize any C-tag fusion protein
Applications for CaptureSelect™ Biotin Anti-C-tag Conjugate include ELISA, Western blot, Gyrolab®-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore® and IBIS-MX96 systems) and bio-layer interferometry (ForteBio® Octet® systems).
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For Research Use Only. Not for use in diagnostic procedures.