Peptide corresponding to amino acids 561–570 of human Metadherin
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
1-3µg x 10^6 cells
Immunocytochemistry (ICC)
1-3 ug/ml
Immunofluorescence (IF)
1-3 ug/ml
Immunohistochemistry (Paraffin) (IHC (P))
1:10-1:100
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
Metadherin (MTDH) was originally identified as an oncogene, and plays a role in promoting proliferation, invasion, survival, and enhancing chemoresistance. Expression of MTDH is associated with Ha-ras, PI3K/Akt, NF- kappaB and Wnt/ beta-catenin signaling pathways. Immunoflurescence and histochemical analysis has shown perinuclear, nucleolar, and cytoplasmic staining, while upregulated MTDH expression has been shown to enhance nuclear localization. The protein has three putative lysine-rich nuclear localization signals and this antibody has been raised specifically against the NLS3 region.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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