Peptide corresponding to amino acids 502–515 of human VPS34
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Immunocytochemistry (ICC)
1-3 ug/ml
Immunofluorescence (IF)
1-3 ug/ml
Immunohistochemistry (Paraffin) (IHC (P))
1:50
Western Blot (WB)
2-5 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
VPS34 is a class III phosphatidylinositol 3-kinase (PI3K). PI3K are lipid kinases that regulate vital cellular processes, including signal transduction, membrane trafficking, cytoskeletal regulation and apoptosis. VPS34 plays a role in insulin-stimulated mitogenesis. It is required for internal vesicle formation within multivesicular endosomes. VPS34 and its adapter protein p150 are Rab7 interacting partners, and the hVPS34/p150 complex co-localizes with Rab7 on late endosomes.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.