Recombinant Protein corresponding to amino acids 378-525 of human PDI (ER Marker).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Western Blot (WB)
1-2 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
PDI (ER marker) also known as protein disulfide isomerase is an enzyme present in the ER which plays a very important role in protein folding, by facilitating formation of correct disulphide bonds. Processing and maturation of the secretory proteins in the endoplasmic reticulum are actively done by the family of PDI proteins. This class of proteins show similar homology to the cytoplasmic protein thioredoxin. The protein catalyses the thiol/disulphide interchange interactions. Also depending upon the type of polypeptide substrate and redox potential, the protein brings about protein disulfide formation, isomerization or reduction.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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