Peptide corresponding to amino acids 173–184 of human SHH.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
1 ug/ml X 10^6 cells
Immunocytochemistry (ICC)
0.5 ug/ml
Immunofluorescence (IF)
0.5 ug/ml
Western Blot (WB)
2-3 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
This antibody is predicted to react with canine, non-human primate, mouse, rabbit, rat and porcine based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
Sonic Hedgehog (SHH) is a secreted signaling protein belonging to Hedgehog family. It plays an important role in patterning and organogenesis during embryonic development. In adult cells, SHH plays a role in control of cell division. The hedgehog signalling pathway is initiated when SHH binds the Patched receptor (PTC) resulting in release of SMO from repression. SMO acts to inhibit proteolytic cleavage of the Ci protein which acts as a transcriptional activator.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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