Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
2-4µg x 10^6 cells
Immunocytochemistry (ICC)
2-4µg
Immunofluorescence (IF)
2-4µg
Western Blot (WB)
0.5-1µg/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
ERK-1 is a classical MAP kinase involved in intracellular signaling in response to extracellular stimulus. ERK-1 is activated by growth factors, hormones, and phorbol esters. ERKs are widely expressed in different cells, and are involved in control of cell proliferation, cell differentiation, and cytoskeletal regulation. ERK-1 is a serine/threonine kinase which phosphorylates cytoplasmic signaling molecules, cell surface receptors, transcription factors, and microtubule associated proteins upon activation by molecules upstream in the signal transduction pathway.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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